EXTERACELLULAR L-ASPARAGINASE FROM LOCAL SOIL BACTERIA: PRODUCTION AND OPTIMIZATION OF PHYSIOLOGICAL CONDITION

Orabi, Hanaa M.; El-Fakrany, Esmail M.; Abdel Khalek, Eman S.; El-Kasaby, Aziza M.; Sidkey, Nagwa M.

doi:10.21608/absb.2018.33806

EXTERACELLULAR L-ASPARAGINASE FROM LOCAL SOIL BACTERIA: PRODUCTION AND OPTIMIZATION OF PHYSIOLOGICAL CONDITION

Document Type : Original Article

Authors

¹ Botany & Microbiology Department, Faculty of Science, Al-Azhar University (Girls Branch)

² Protein Research Department, Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technology Applications, Alexandria, Egypt.

10.21608/absb.2018.33806

Abstract

L-asparaginase produced by Bacillushalotolerans OHEM18 finds broad applications due to its hydrolysis activity so used as therapeutic agents in combination with additional medications in the cure of acute lymphoblastic leukemia and also in food processing. In the present study, L-asparaginase production was optimizedand the optimum factors for production under submerged fermentation (SMF) of the asparaginase were pH 7.0 after 28h using glucose and asparagine as carbon and nitrogen sources, respectively while bagasse was the best inducer for L-asparaginase production under SSF.

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