TY - JOUR ID - 33806 TI - EXTERACELLULAR L-ASPARAGINASE FROM LOCAL SOIL BACTERIA: PRODUCTION AND OPTIMIZATION OF PHYSIOLOGICAL CONDITION JO - Al-Azhar Bulletin of Science JA - ABSB LA - en SN - 1110-2535 AU - Orabi, Hanaa M. AU - El-Fakrany, Esmail M. AU - Abdel Khalek, Eman S. AU - El-Kasaby, Aziza M. AU - Sidkey, Nagwa M. AD - Botany & Microbiology Department, Faculty of Science, Al-Azhar University (Girls Branch) AD - Protein Research Department, Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technology Applications, Alexandria, Egypt. Y1 - 2018 PY - 2018 VL - 29 IS - Issue 2-C SP - 1 EP - 7 KW - L-asparaginase KW - enzyme production KW - submerged fermentation and solid state fermentation DO - 10.21608/absb.2018.33806 N2 - L-asparaginase produced by Bacillushalotolerans OHEM18 finds broad applications due to its hydrolysis activity so used as therapeutic agents in combination with additional medications in the cure of acute lymphoblastic leukemia and also in food processing. In the present study, L-asparaginase production was optimizedand the optimum factors for production under submerged fermentation (SMF) of the asparaginase were pH 7.0 after 28h using glucose and asparagine as carbon and nitrogen sources, respectively while bagasse was the best inducer for L-asparaginase production under SSF. UR - https://absb.journals.ekb.eg/article_33806.html L1 - https://absb.journals.ekb.eg/article_33806_5a3d8c047d321f04374729fe85273f99.pdf ER -