EFFECT OF GAMMA IRRADIATION ON CELLULASE ACTIVITY OF PLEUROTUS PULMONARIUS DURING THE DIFFERENT GROWTH STAGES ON DIFFERENT PLASTIC POLYETHYLENE WASTES

Document Type : Original Article

Authors

1 Microbiology department,Faculty of Science ,Ain shamsUniversity,Egypt

2 Microbiology department,Faculty of Science ,Ain shams University, Egypt

3 Radio-Biochemistry, Atomic Energy Authority, Egypt

4 Microbiology department, Faculty of Science ,Ain shams University,Egypt

5 Microbiology department, Middle Eastern Regional Radioisotope center for the Arab Countries, Egypt.

Abstract

Pleurotus pulmonarius was excellent growth on determined weights of the wet rice straw, non irradiated plastic, irradiated
plastic, mixture of rice straw and non irradiated plastic and mixture of rice straw and irradiated plastic. maximum value of
cellulase recorded on rice straw, mixture of rice straw and irradiated plastic, mixture of rice straw and non- irradiated plastic (1.7, 1.5 and 1.3 unit/gm waste), respectively, which recorded after first harvest; but irradiated plastic and non-irradiated plastic giving highest values at promordium stage; (1.14 and 0.79 unit/gm waste). The spawn of P. pulmonarius was irradiated at the does 0.5, 1 and 2 KGy, and inoculated on each different wastes separately to detect the cellulase activity which was increased in the various wastes by exposing the spawn of P. pulmonarius to gamma irradiation and detectable raises in the cellulase activity was recorded at dose 0.5 KGy, Any further increase in the
irradiation dose was accompanied by a decrease in cellulase activity until reach lowest value at 2 KGy.

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