Amelioration of Sodium Nitrate-Induced kidney and Lipid Disorders thru Combination of Silver Nanoparticles with Wheat Germ Oil, InVitro/InVivo

Document Type : Original Article


1 Department of nutritional chemistry and metabolism, National Nutrition Institute, Cairo, Egypt.

2 Department of Chemistry, Faculty of Science (Girls), Al-Azhar University, Cairo, Egypt.

3 Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, 11884, Cairo, Egypt.

4 Pre-Treatment and Finishing of Cellulosic Fabric Department, Institute of Textile Research and Technology, National Research Center, 33- El- Buhouth St, Dokki, Cairo, 12622.


Nanomaterial may aid in improving the human quality of life. This study supposed silver nanoparticles (Ag NPs) and" wheat germ oil (WGO)" eliminate the negative consequences of sodium nitrate (NaNO3) on kidney and lipid disorders. Ag NPs were synthesized with two concentrations (10, 50 ppm). Spectroscopic techniques such as UV–Vis, X-ray diffraction (XRD), atomic force microscopy (AFM), and dynamic light scattering (DLS) were extensively used for the characterization of the Ag NPs. They had an average size of 16.8 nm, a poly disparity index (PDI) of 0.3, and a zeta potential (ZP) of -34.8. Bacillus subtilis (ATCC 6633) was utilised as the positive bacteria, and Salmonella typhimurium (ATCC 14028) was employed as the negative bacteria in this study.  Combination of WGO, and Ag NPs showed an antibacterial effect reaching 91%, and 80% on Salmonella typhimurium and  Bacillus subtilis  respectively with a non-significant effect of both Ag NPs concentrations. Moreover, 70 male albino rats were separated into seven groups: G1 control, G2 positive control received NaNO3 orally15 mg/kg, G3 ate "WGO" only, and G4 fed Ag NPs 10ppm. Treatment groups (G5, G6, and G7) intake orally NaNO3 15 mg/kg with WGO, Ag NPs10ppm, and a combination of WGO and AgNPs10ppm respectively. In the positive control group were appeared; the body weight gain (BWG) and feed intake (FI) are decreased, increasing in renal function resulting from lipid profile syndrome, oxidative stress biomarker showed increasing in malondialdehyde (MDA), decreasing in glutathione (GSH), and catalase enzyme (CAT).  However, all of these harmful impacts generated by NaNO3 were dramatically reduced in the treated groups. The research managed to combine wheat germ oil and symbiosis Ag NPs diminished the preceding parameter, and that the histological structure of the kidney in the treatment groups was restored to normal group.


Main Subjects

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