Elsharnoby, H., Kandil, O., Abu-Elnaga, H. (2021). Dromedary camel epididymal sperm characteristics at breeding and non-breeding seasons. Al-Azhar Bulletin of Science, 32(Issue 1-C), 1-9. doi: 10.21608/absb.2021.67232.1104
H. Elsharnoby; O. M. Kandil; H. A. Abu-Elnaga. "Dromedary camel epididymal sperm characteristics at breeding and non-breeding seasons". Al-Azhar Bulletin of Science, 32, Issue 1-C, 2021, 1-9. doi: 10.21608/absb.2021.67232.1104
Elsharnoby, H., Kandil, O., Abu-Elnaga, H. (2021). 'Dromedary camel epididymal sperm characteristics at breeding and non-breeding seasons', Al-Azhar Bulletin of Science, 32(Issue 1-C), pp. 1-9. doi: 10.21608/absb.2021.67232.1104
Elsharnoby, H., Kandil, O., Abu-Elnaga, H. Dromedary camel epididymal sperm characteristics at breeding and non-breeding seasons. Al-Azhar Bulletin of Science, 2021; 32(Issue 1-C): 1-9. doi: 10.21608/absb.2021.67232.1104
Dromedary camel epididymal sperm characteristics at breeding and non-breeding seasons
1Embryology, Zoology and Entomology Department, Faculty of Science, Al-Azhar University (Girls).
2Embryo Biotechnology, Department of Animal Reproduction & Artificial Insemination, Veterinary Research Division, National Research Centre
Abstract
Testicles from 30 clinically healthy male mature dromedary camels were gathered from the El-Basatin slaughterhouse all the breeding season (Nov. 2018 to Apr. 2019) and the nonbreeding season (May 2019 to Oct. 2019). Within one year the epididymal sperm were obtained twice per month. This manuscript aimed to investigate the influence of seasonality on dromedary camel epididymal sperm characteristics (motility, viability, sperm cell concentration, morphology and membrane integrity). The results depicted that during the breeding period, the rate of motility, concentration, live spermatozoa, normal morphology and the intact membrane of dromedary camel epididymal sperm showed a high significant increase (P<0.05) when compared to the nonbreeding period. While rate of death, abnormalities and membrane damage of the dromedary camel epididymal sperm were highly increased (P <0.05) during non-breeding when compared to the breeding period. In conclusion, the dromedary epididymal camel’s sperm characteristics, viability and quality improved during breeding season.
[1] Skidmore JA, Reproductive physiology in female old world camelids Anim. Reprod. Sci. 2011; 124: 148–154 doi: 10.1016/j.anireprosci.2010.08.023. Epub 2010 Sep 3.
[2] Hemeida NA, Development and application of the reproductive technologies to camel breeding. Proceedings of the International Camel Conference, Al-Hasa, Saudi Arabia. Camel Publishing House) 2015; pp: 367–373.
[3] Koivisto MB, Costa MTA, Perri S and Vicente WRR, The effect of season on semen characteristics and freezability in Bos indicus and Bos taurus bulls in the southeastern region of Brazil. Reprod. Domest. Anim. 2009; 44: 587–592 DOI: 10.1111/j.1439-0531.2008. 01023.
[4] Bhakat M, Mohanty TK, Gupta AK, Prasad S, Chakravarty AK, and Khan HM, Effect of season on semen quality parameters in Murrah buffalo bulls. Buffalo Bull. 2015; 34 100–112.
[5] Zarazaga LA, Guzmán JL, Domínguez C, Pérez MC, and Prieto R, Effects of season and feeding level on reproductive activity and semen quality in Payoya buck goats. Theriogenology, 2009, 71: 1316–1325 DOI: 10.1016/j.theriogenology.2009.01.007 .
[6] Gamboa S, Rodrigue AS, Henriques L, Batist C, and Ramalho-Santos J, Seasonal functional relevance of sperm characteristics in equine spermatozoa. Theriogenology, 2010, 73: 950–958 DOI:10.1016/j.theriogenology.2009.11.023
[7] Malejane CM, Greyling JPC, and Raito MB, Seasonal variation in semen quality of Dorper rams using different collection technique. S. Afr. J. Anim. Sci., 2014; 44 :26–32.
[8] El-Kon II, Heleil BA, and Mahmoud SA, Effect of age and season on the testicular sperm reserve and testosterone profile in camel (Camelus dromedarius). Anim. Repro., 2011; 8: 68–7 DOI: 10.13140/RG.2.1.3627.7921
[9] Al-Bulush S, Manjunatha M, De Graaf S, Rickard JP, Reproductive seasonality of male dromedary camels . Anim. Reprod. Sci, 2019; 202: 10–20 https://doi.org/10.1016/j. anireprosci.2018.12.013.
[10] Swelum, AA, Saadeldin, IM, Ba-Awadh H, and Alowaimer AN, Effects of melatonin implants on the reproductive performance and endocrine function of camel (Camelus dromedarius) bulls during the non-breeding and subsequent breeding season. Theriogenology. 2018; 119: 18–27 DOI: 10.1016/j. theriogenology.2018.06.017 .
[11] Escoffier JAB, Kaba M, Hograindleur JP, Le Bléve E, Martinez G, Stévant I, Ra PF, Arnoul C, and Ne S, Pantoprazole, a proton‐pump inhibitor, impairs human sperm motility and capacitation in vitro. Andrology. 2020; 8: 1795–1804 DOI: 10.1111/andr.12855 .
[12] Turri FK, Abdoon AS, Sabra H, El Atrash, and Piz F, Conservation of camel genetic resources: epididymal sperm recovery. The Camel Conference. SOAS ., 2013; 29: 27–32.
[13] Goto K, Kajihara Y, Kob M, Kosaka S, Nakanishi Y, and Ogaw K, In vitro fertilization and development of in vitro matured bovine follicular oocytes J. Anim. Sci., 1989; 6: 2181–2185 doi: 10.2527/jas1989. 678218.
[14] Turri F, Madeddu M, Gliozzi TM, Gandini G, and Pizzi F, Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve genebanking in local breeds. Animal, 2014; 8: 440–447 doi: 10.1017/S1751731113002279..
[15] Turri F, Madeddu M, Gliozzi M, Gandini G, and Pizzi F, Influence of recovery methods and extenders on bull epididymal spermatozoa quality. Reprod. Domest. Anim., 2012; 47 712–47 719 doi: 10.1111/j.1439-0531.2011.01948..
[16] Maiada WA, Allam AEB, Zeidan A, Farouk MH and Abd El-Salaam M, Morphological and histological changes in the camel testes in relation to semen characteristics during breeding and non-breeding seasons. J. An. Sci., 2013; 9:74–8.
[17] Louis KS, and Siegel AC, Cell viability analysis using trypan blue: manual and automated methods. Mammalian Cell Viability 2011; 7–12.DOI.10.1007-978-1-61779-108-6_2
[18] Gradinarska D, Ivanova-Kicheva M, Daskalova D, Lazov K, and Chervenkov M, Comparative analysis of the capacitation ability of fractionally obtained canine spermatozoa. J. Mt. Agric. Balk. 2015;18: 412–425.
[19] Monaco D and Lacalandra GM, Considerations for the development of a dromedary camel (Camelus dromedarius) semen collection centre. Anim. Reprod. Sci., 2020; 212: 106239-106245 doi: 10.1016/j.anireprosci.2019.106239. Epub 2019 Nov 18.
[20] Gherissi DE, Afri-Bouzebda, and Bouzebda Z, Seasonal changes in the testicular morphology and interstitial tissue histomorphometry of Sahraoui camel under Algerian extreme arid conditions Biol. Rhythm Res., 2018; 49: 291–301.
[21] Abd El-Samee AM, Zeida A, Abbas HE, and Ahmadi E, Semen quality and testicular histology with special reference to different seasons of the year and ages of the male dromedary camel. Proc. Intern. Scientific Conf. Camels.Qassim Univ.KSA. 2006
[22] Ibrahim M, and Abd-El-Rahman HM, Studies on some biochemical, hormonal, histopathological and seminal characters in relation to rutting and non-rutting season in camels. Alexandria J. Vet. Sci.,2016; 49: 189–202.
[23] Giuliano S, Director A, Gambarotta M, Trasorras V, and Miragaya M, Collection method, season and individual variation on seminal characteristics in the llama (Lama glama) Anim. Reprod. Sci., 2008; 359–369 doi: 10.1016/j.anireprosci.2007.02.016.
[24] Agarwal VK, Ram L, Rai AK, Khanna ND, and Agarwal S, Physical and biochemical attributes of camel semen. J. Cam. Res.,2004;1: 25-33.
[25] Gatimel N, Mansoux L, Moreau J, Parinaud J, and Léandr D, Continued existence of significant disparities in the technical practices of sperm morphology assessment and the clinical implications.Fertil. Steril., 2017;107: 365–372 doi: 10.1016/j.fertnstert.2016.10.038.
[26] El-Harairy M, Zeidan A, Afify A, Amer H, and Amer A, Ovarian activity, biochemical changes and histological status of the dromedary she-camel as affected by different seasons of the year. Nat. Sci.,2010; 8: 54–65.
[27] Shekhe C, Vya S, Purohi G, and Patil N, Use of collagenase type-1 to improve the seminal characteristics of dromedary camel semen. Eur. J. Vet. Med.,2012;1: 17–27.
[28] Buendıa P, Sole C, Paolicchi F, Gago G, Urquieta B, Pérez-Sánchez F, and Bustos-Obregón E, Morphometric characterization and classification of alpaca sperm heads using the Sperm-Class Analyzer® computer-assisted system. Theriogenology 2002; 57 1207–1218 DOI: 10.1016/s0093-691x(01)00724-5.
[29] Zeidan A, Habeeb AAM, Ahmadi E, Amer H, and Abd El-Razik A, Testicular and physiological changes of the male dromedary camels in relation to different ages and seasons of the yea. Proceedings of 2nd International Conference on Animal Production and Health in Semi-arid Areas, El-Arish, North Sinai, Egypt. 2001; pp: 147-16.
[30] Abdallah,LA, Ovulation induction and insemination with fresh semen in the one-humped camel (Camelus dromedaries) in non–breeding season. Ph.D.Sudan Univ.of Science and Technology, 2015.
[31] Martí JI, Aparicio IM, Leal C, and García-Herreros M, Seasonal dynamics of sperm morphometric subpopulations and its association with sperm quality parameters in ram ejaculates. Theriogenology,2012; 78: 528–541 doi: 10.1016/j.theriogenology.2012.02.03.
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