• Home
  • Browse
    • Current Issue
    • By Issue
    • By Author
    • By Subject
    • Author Index
    • Keyword Index
  • Journal Info
    • About Journal
    • Aims and Scope
    • Editorial Board
    • Publication Ethics
    • Peer Review Process
  • Guide for Authors
  • Submit Manuscript
  • Reviewers
  • Contact Us
 
  • Login
  • Register
Home Articles List Article Information
  • Save Records
  • |
  • Printable Version
  • |
  • Recommend
  • |
  • How to cite Export to
    RIS EndNote BibTeX APA MLA Harvard Vancouver
  • |
  • Share Share
    CiteULike Mendeley Facebook Google LinkedIn Twitter
Al-Azhar Bulletin of Science
arrow Articles in Press
arrow Current Issue
Journal Archive
Volume Volume 32 (2021)
Volume Volume 31 (2020)
Volume Volume 30 (2019)
Volume Volume 29 (2018)
Volume Volume 28 (2017)
Volume Volume 27 (2016)
Volume Volume 26 (2015)
Volume Volume 25 (2014)
Volume Volume 24 (2013)
Volume Volume 23 (2012)
Volume Volume 22 (2011)
Volume Volume 21 (2010)
Volume Volume 20 (2009)
Volume Volume 19 (2008)
Volume Volume 18 (2007)
Issue Issue 2-C
Issue Issue 2-A
Issue Issue 1-A
Issue Issue 1-C
Volume Volume 17 (2006)
Volume Volume 16 (2005)
OMRAN, A. (2007). SIMULTANEOUS DETERMINATION OF HYDROCHLOROTHIAZIDE AND METHYLDOPA IN TABLETS USING FIRST DERIVATIVE SPECTROPHOTOMETRY AND PLS. Al-Azhar Bulletin of Science, 18(Issue 2-A), 113-126. doi: 10.21608/absb.2007.11123
AHMED A. OMRAN. "SIMULTANEOUS DETERMINATION OF HYDROCHLOROTHIAZIDE AND METHYLDOPA IN TABLETS USING FIRST DERIVATIVE SPECTROPHOTOMETRY AND PLS". Al-Azhar Bulletin of Science, 18, Issue 2-A, 2007, 113-126. doi: 10.21608/absb.2007.11123
OMRAN, A. (2007). 'SIMULTANEOUS DETERMINATION OF HYDROCHLOROTHIAZIDE AND METHYLDOPA IN TABLETS USING FIRST DERIVATIVE SPECTROPHOTOMETRY AND PLS', Al-Azhar Bulletin of Science, 18(Issue 2-A), pp. 113-126. doi: 10.21608/absb.2007.11123
OMRAN, A. SIMULTANEOUS DETERMINATION OF HYDROCHLOROTHIAZIDE AND METHYLDOPA IN TABLETS USING FIRST DERIVATIVE SPECTROPHOTOMETRY AND PLS. Al-Azhar Bulletin of Science, 2007; 18(Issue 2-A): 113-126. doi: 10.21608/absb.2007.11123

SIMULTANEOUS DETERMINATION OF HYDROCHLOROTHIAZIDE AND METHYLDOPA IN TABLETS USING FIRST DERIVATIVE SPECTROPHOTOMETRY AND PLS

Article 8, Volume 18, Issue 2-A, December 2007, Page 113-126  XML PDF (273.35 K)
Document Type: Original Article
DOI: 10.21608/absb.2007.11123
Author
AHMED A. OMRAN email
Chemistry Department, Faculty of Science, Al-Azhar University, Assiut 71524, Egypt
Abstract
Two new analytical methods are described as
convenient and useful alternatives for simultaneous determination of
hydrochlorothiazide (HCTZ) and methyldopa (MD) in their combinations. The first
method depends on the first order derivative spectrophotometry by measuring the
amplitudes at zero-crossing wavelengths of 286 and 270 nm for HCTZ and MD,
respectively. In the second method, partial least squares (PLS) analysis of the
ultraviolet absorption spectra of the samples in the 240-340 nm region was
applied. The methods were calibrated between 3.5 and 35.7 mg mL-1 for HCTZ and between 2.5 and 25.3 mg mL-1 for MD. For PLS chemometric
calibration a concentration set of random mixture consisting of the two drugs
in ethanol was prepared. The absorbance data in UV spectra and their dA/dl values were measured for the 24 l points considering Dl= 4 nm. The calibration of the PLS method involves
both absorbance-concentration and dA/dl-concentration data matrices. The numerical values
were calculated by using Matlab version 6.5 and origin 7.0 software. The
results of the methods were statistically compared with each other and a good
harmony was found. The developed calibrations were successfully applied for
assaying the pharmaceutical formulation of AldorilÒ tablets
Keywords
Hydrochlorothiazide; Methyldopa; Aldoril tablets; derivative spectrophotometry; PLS
Main Subjects
Chemistry
Statistics
Article View: 138
PDF Download: 190
Home | Glossary | News | Aims and Scope | Sitemap
Top Top

Journal Management System. Designed by NotionWave.