2024-03-29T07:51:13Z
https://absb.journals.ekb.eg/?_action=export&rf=summon&issue=2050
Al-Azhar Bulletin of Science
1110-2535
1110-2535
2008
19
Issue 1-C
PROTECTIVE ROLE OF MELATONIN ON SOME BIOCHEMICAL ASPECTS OF NICOTINE ADMINISTRATION ON BRAIN OVARIECTOMIZED FEMALE RATS
AMIRA
MERSAL
The protective effect of melatonin against the damage of nicotine on the brain in ovariectomized female rats (OVX) was investigated. Cigarette smoking is common in societies worldwide and has been identified as injurious to human health. The data revealed that nicotine,a major component of cigarette smoke ( at a dose of 2 mg/ KG b.wt.) resulted in a significant increase in total proteins, lipid peroxides (LPO), total cholesterol and phospholipids, and a significant decrease in acetylcholenesterase activity (AChE) and glutathione content (GSH).
Melatonin administration at dose (5 mg/kg b.wt.)to nicotine treated rats showed significantly ameliorated changes in total proteins, acetylcholenesterase (AChE) , glutathione content (GSH) , lipid peroxides (LPO) , total cholesterol (TC) and phospholipids (Ph). The obtained results suggest that the protective effect of melatonin is mediated through decreased oxidation of lipids , thus minimizing the risk posed by nicotine .
Melatonin
Nicotine
Brain
2008
06
01
1
13
https://absb.journals.ekb.eg/article_10496_1ae59654ca38ecefacd18541fbec66d1.pdf
Al-Azhar Bulletin of Science
1110-2535
1110-2535
2008
19
Issue 1-C
PREVENTION OF ALUMINIUM TOXICITY AND OXIDATIVE DAMAGE BY MELATONIN IN BRAIN OVARIECTOMIZED FEMALE RATS
AMIRA
MERSAL
Aluminium (AL) is an important element with known toxicity in human body, mainly in the centeral nervous system. Melatonin is known as antioxidant, free radical scaverger and metal chelator. The aim of this study to determine the effect of oral administration of melatonin on accumulation and toxicity of Alclз in brain of ovariectomized female rats. Orally administerated of Alcl3 at dose (10mg/Kg b.w.) for 8 weeks showed significant decrease in total protein,acetylcholenesterase(AchE) and glutathione content in brain tissues. While the lipid peroxidation Lpo, triglycerides Trig, total cholesterol and phospholipids showed significant Increase. The treatment with melatonin at dose (5mg/kgb.w.) for 8 weeks showed slightly decreased pervious parameters.These data indicated that orally treatment with melatonin against Alcl3 is probably responsible for preventaion of oxidative damage and Al-toxicity induced injury to brain of experimental rats.
Aluminium toxicity
oxidative damage
Brain
2008
06
01
15
26
Al-Azhar Bulletin of Science
1110-2535
1110-2535
2008
19
Issue 1-C
PROTECTIVE EFFECT OF Β-CAROTENE ON GAMMA RADIATION- INDUCED RENAL HISTOLOGICAL AND ULTRASTRUCTURAL CHANGES IN MALE ALBINO RATS
SEHAM
ABU-NOUR
MOHAMED
RADY
IHAB
KHALIL
The present work was conducted to investigate the histological and ultra-structural changes in the kidney tissues of male albino rats due to the exposure of gamma-radiation and to investigate tissue-protection from the damaging effects of gamma-radiation using a natural product namely, Beta-carotene.
The histological studies revealed progressive pathological lesions in the kidney of whole body γ-irradiated rats; as represented by severe damage in glomerular tuft and increase in masengal cells with wide Bowman's space and sever hemorrhagic areas between degenerated renal tissue, andrenal tubules exhibited various degrees of degeneration. The ultra-structural studies revealed that epithelial cells lining the proximal and distal convoluted tubules exhibited an increase in the swelling mitochondria, dilation in the rough endoplasmic reticulum and electron dense in the nuclear chromatin. Thickness in basement membrane, dilation of infolded, and damage of brush border of the proximal tubules were also observed. The treatment with β-carotene pre-exposure to gamma radiation attenuated most of these changes. Therefore, the present study has implication for the potential use of β-carotene as a radioprotector.
Protective Effect
Β-CAROTENE
gamma radiation
male Albino rats
2008
06
01
27
44
https://absb.journals.ekb.eg/article_10498_b3b9a54a65e52b1e2b4ceba807c98cbe.pdf
Al-Azhar Bulletin of Science
1110-2535
1110-2535
2008
19
Issue 1-C
PURIFICATION AND EXTRACTION OF POLYSACCHARIDE SPECIFIC OF STREPTOCOCCUS PYOGENES OF GROUP A FIXING ON LATEX PARTICLES
AHMED
ATIA
HUSSEIN
EL-KABBANI
The Streptococci are widely distributed in nature and frequently form part of the normal human flora which are members. Approximately 5-15% of humans carry Streptococcus pyogenes. Cytoplasmic membrane has antigens similar to those of human cardiac and heart valve fibroblasts. Patients may also develop immune- mediated such as acute rheumatic fever , skin infections and endocarditis. S. pyogenes evolved anenzyme that specifically targets IgG use as biochemical reactions, and serologic specificity. Serologic grouping is based on antigenic differences in cell wall carbohydrates (groupA). A technique for detection of antibodies against S. pyogensgroup antigen / type antigens is being devised as one of the procedures possibly useable in prevention of infections and manufacturers in development of diagnostic and identification tools. Method used for detection of antibodies ( latex agglutination ) are being standardised with antibody levels has been introduced. The detection of antibodies induced by conjugated is under development for antibiotic resistance of Streptococcus pyogenes prepared and provided freeze-dried reference type cultures of Streptococci. The stability of the obtained component subjected at 4 °C and 37 °C over one period to reveal several groups of serum representing healthy population ( N = 20 ) and patients ( N = 40 ) as a significant correlation for the diagnostic of acute rheumatic arthritis .We have shownthat the total amount of specific antibodies does not necessary correlate with the level of neutralizing antibodies, and antibody quality might be clinically more important than antibodyquantity
Purification
EXTRACTION OF POLYSACCHARIDE SPECIFIC OF STREPTOCOCCUS PYOGENES OF GROUP A FIXING ON LATEX PARTICLES
2008
06
09
43
58
https://absb.journals.ekb.eg/article_10499_6eb67b086ff1e738671fd40e58b20c1f.pdf
Al-Azhar Bulletin of Science
1110-2535
1110-2535
2008
19
Issue 1-C
ANTIBACTERIAL ACTIVITY OF ROOT EXTRACTS OF RUMEX NERVOSUS GROWING IN YEMEN
ESAM
HUSSEIN
AHMED
AL-HAZMI
ESAM
AQLAN
SAMIRA
AL-GA'AFARY
Roots of Rumex nervosus growing in the mountains around Ibb university, Yemen Republic, were collected in February 2008, washed under tap water, cut into small pieces and dried using hot air oven at 60oC until a constant weight was obtained. The dried plant material was then ground into a fine powder and successively extracted till exhaustion with petroleum ether 60/80°C, diethyl ether, chloroform, acetone and methanol. Extraction was carried out by cold soaking to avoid breakdown of active principles. The obtained extracts yielded gummy materials after gentle drying. Filter paper discs were impregnated with 10 mg of each of the extracts obtained and the antibacterial activity was checked by the disc-plate diffusion method. The results obtained have revealed that except for the methanol extract, all the other extracts exhibited varying antibacterial activities against the tested pathogenic bacteria. The obtained results indicate that Rumex nervosus growing in Yemen can be used in future as a source of active antimicrobial agents for industry and medicine.
Rumex nervosus
Antibacterial
Successive extraction
2008
06
01
45
51
https://absb.journals.ekb.eg/article_10500_bb67dbd86f97de69ee846cab14dd87b8.pdf
Al-Azhar Bulletin of Science
1110-2535
1110-2535
2008
19
Issue 1-C
IDENTIFICATION OF SOME BACILLUS ISOLATES PRODUCING EXO-ENZYMES USING QUANTITATIVE REAL-TIME POLYMERASE CHAIN REACTION (PCR)
E.
MAKKY
M.
MUHARRAM
R.
BAYOUMI
The basic goal of this study is the isolation of some Bacillus isolates from different sources for production of bioactive products such as detection of cellulose(s) and pectinase(s) and identification of these isolates using quantitative real-time PCR (qRT PCR) depending on 16S rRNA gene in each isolate. Nine, six and two isolates were obtained from municipal sewage water, composted rice straw and expired pharmaceutical drugs (Capozide), respectively. Out of nine isolates, only four Bacilli (SW2, SW3, SW6 and SW9) were selected and examined for cellulose(s) and pectinase(s) production, and revealed that only SW2 and SW9 exhibited maximum pectate lyase (PL) and polygalacturonase (PGase) viz. 465.55 and 222.53 (U/mg), respectively. Out of six Bacilli isolates (RS1, RS3 and RS5), only RS3 showed maximum productivity of carboxymethylcellulase (CMCase) viz. 657.21 (U/mg). On the other hand, only one Bacillus isolate out of two was selected ECC2 and exhibited the maximum Avicelase productivity viz. 533.32 (U/mg). TaqMan qRT PCR method was used to detect and quantify the 16S rRNA genes of Bacillus isolates tested. Forward primer Bsub5 F and the reverse primer Bsub3 R were used for the amplification of 16S rRNA of the “Bacillus subtilis group”, it is also successful to demonstrate the most similarities of the Bacillus isolates in this study and they are closely related to “Bacillus subtilis group”.
Polygalacturonase
Pectin Lyase
Avicelase
Carboxymethylcellulase
quantitative real-time PCR
2008
06
01
53
67
https://absb.journals.ekb.eg/article_10501_304b9fa3fdeb4bd2955950a31fc9c934.pdf