Al-Azhar University, Faculty of Science (Boys)
Al-Azhar Bulletin of Science
1110-2535
2636-3305
17
Issue 2-C
2006
12
20
STUDIES OF SEED PROTEIN, ESTERASE ISOZYME AND SEED COAT SCAN ON SOME CULTIVATED SPECIES OF GENUS MINUSOPS (SAPOTACEAE) IN EGYPT
21
32
EN
WAEL
T.
KASEEM
Botany and Microbiology Department, Faculty of Science, Al- Azhar University, Madient Nasr, Cairo, Egypt.
MAHMOUD
A.
MONSOUR
Botany and Microbiology Department, Faculty of Science, Al- Azhar University, Madient Nasr, Cairo, Egypt.
10.21608/absb.2006.14442
Electrophoretic profiles of native seed protein and esterase isozyme characters of four species of genus <em>Mimusops </em>have been revealed by PAGE. Seed coat scanning and numerical analysis of the results carried out. These species are <em>M</em>.<em> laurifolia</em>, <em>M</em>.<em> elengi</em>,<em>M</em>.<em> zeyheri</em> and <em>M</em>.<em> coffra</em>. The results of SEM on the surface seeds showed that, Seed ornamentation in <em>Mimusops zeyheri</em> differs than the rest species of the <em>genus Mimusops</em> by showing replicas of freeze on the their membrane surface. The results of seed protein and esterase isozyme characters indicated that delimitation of <em>Mimusops zeyheri</em> in a separated level, separation of two species of <em>Mimusops coffra</em> and <em>Mimusops elengi</em> in a separated group, in addition distinguishing of <em>Mimusops laurifolia</em> than other two species of <em>Mimusops elengi </em>and<em> Mimusops coffra.</em>
seed protein,ESTERASE ISOZYME,SEED COAT SCAN,GENUS MINUSOPS
https://absb.journals.ekb.eg/article_14442.html
https://absb.journals.ekb.eg/article_14442_3629b2dd04be8b094b7f1f659619f8c5.pdf
Al-Azhar University, Faculty of Science (Boys)
Al-Azhar Bulletin of Science
1110-2535
2636-3305
17
Issue 2-C
2006
12
01
GROWTH AND LEAF PROTEINS OF FENUGREEK PLANTS (TRIGONELLA FOENUM GRAECUM) GROWN UNDER THE EFFECT OF WATER STRESS AND TREATMENT WITH SOME GROWTH REGULATORS
33
46
EN
E.
A.
HUSSEIN
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.
M.
A.
AL-SHEMAIRY
Biology Department, Sana'a University, Sana'a, Yemen Republic.
10.21608/absb.2006.14444
In the present study, Fenugreek (<em>Trigonella foenum graecum</em>) seedlings were divided into three groups, the first of which was allowed to grow under normal irrigation conditions (full field capacity), the second under moderate water stress (50 % of the field capacity) and the third under severe water stress (25 % of the field capacity). With the beginning of stress treatment, the seedlings of each group were subdivided into four subgroups. The first represented control, the second sprayed with GA<sub>3</sub> (1000 p.p.m), the third with IBA (100 p.p.m) and the fourth with kinetin (100 p.p.m). After two weeks of treatment plants were collected for measurement of plant growth and analysis of leaf proteins to study the effect of water stress and some of the plant growth regulators on growth and gene expression. Some of the growth characters were retarded by water stress. Growth regulators improved the growth of plants under normal irrigation conditions and could overcome some of the adverse effects of stress on water stressed plants. Some alterations on the leaf-protein SDS-PAGE patterns were observed in plants in response to the effect of water stress and the application of growth regulators. GA<sub>3</sub> treatment resulted in the appearance of two novel bands under normal irrigation and disappearance of the stress induced band. IBA resulted in the disappearance of a band that presents in the corresponding control while kinetin resulted in the over-expression of another band.
Water stress,fenugreek,SDS-PAGE,Gene expression,growth hormones
https://absb.journals.ekb.eg/article_14444.html
https://absb.journals.ekb.eg/article_14444_810e61fae9482d02b571b95e76ad45b5.pdf
Al-Azhar University, Faculty of Science (Boys)
Al-Azhar Bulletin of Science
1110-2535
2636-3305
17
Issue 2-C
2006
12
01
EVALUATION OF GENETIC DIVERSITY BETWEEN SOME NITRARIA RETUSA POPULATIONS USING MOLECULAR MARKERS
47
66
EN
Z.
A.
ABO EL-KHEIR
Botany Department, Faculty of of Science, Al-Azhar University (for girls).
M.
O.
EL-MONAYERI
Botany Department, Faculty of of Science, Al-Azhar University (for girls).
D.
ABD EL-RAOF
Botany Department, Faculty of of Science, Al-Azhar University (for girls).
10.21608/absb.2006.14448
<em>Nitraria retusa</em> (Nitrariaceae) is a true xerophyte plant grows in most deserts of Egypt, especially in wadies habitat and dry salt marshes. This study aims to evaluate any possible genetic diversity among samples of <em>Nitraria retusa</em> grown under different habitats using Random Amplified Polymorphic DNA (RAPD) markers, protein electrophoresis and isozyme electrophoresis. For such goal, the ecological aspects of the selected habitats were investigated with the view of finding correlations among these markers and some ecological parameters. Five collected samples of <em>Nitraria retusa</em> were collected from Wadi Hof (sunny and shady), Oyun Musa, Kattamyia and Hammam Faraon. The obtained results showed that differences in the levels of genetic variations detected by the tested markers agree with those observed at the morphological levels. The data obtained from the tested primers resulted in PCR amplified products that differentiate the shady sample of Wadi Hof (with broad leaves) from the other investigated samples. Thus, the results obtained from the present study consider DNA and protein fingerprints for the studied populations.
RAPD markers,protein electrophoresis,isozymes,Ecological parameters,Nitraria retusa
https://absb.journals.ekb.eg/article_14448.html
https://absb.journals.ekb.eg/article_14448_7ff70b5f94df27660646d66b6a46e52f.pdf