Al-Azhar University, Faculty of Science (Boys)
Al-Azhar Bulletin of Science
1110-2535
2636-3305
23
Issue 2-C
2012
12
01
EFFECT OF LIPOPOLYSACCHARIDE AND THE AMELIORATIVE EFFECT OF THE ANTIOXIDANT MELATONIN ON SOME PHYSIOLOGICAL PARAMETERS OF SHEEP EMBRYOS
1
12
EN
DIAA
F.
AHMED
Zoology Department, Faculty of Science, Al -Azhar University, Cairo, Egypt.
SAMIR
A.
ZAAHKOUK
Zoology Department, Faculty of Science, Al -Azhar University, Cairo, Egypt.
EL-YAMANI
I.
EL-ZWAHRY
Zoology Department, Faculty of Science, Al -Azhar University, Cairo, Egypt.
10.21608/absb.2012.7210
<span style="font-size: small;"><span style="font-size: small;">As known, lipopolysaccharide (LPS) is a toxin that is secreted from gram negative bacteria as </span><span style="font-size: small;"><em>E. coli</em></span><span style="font-size: small;"> which is present in the gut of animals like sheep and it may be transported to the embryo through the blood affect the brain and other organs. Little is known about the effect of LPS on liver and kidney function or hematological parameters. In the present study we found that melatonin treatment improved most of the liver functions that affected by LPS injection and also improve kidney function, although LPS injection was found to lead to a decrease of createnine, urea and uric acid for unknown reason. concurrently injection of melatonin plus LPS led to decreasing most of blood indices while increasing of RBCs count and WBCs count. So, we concluded that usage of melatonin as general antioxidant is useful against LPS toxicity.</span></span>
LPS,Ameliorative,Melatonin,kidney,Liver,Blood
https://absb.journals.ekb.eg/article_7210.html
https://absb.journals.ekb.eg/article_7210_fc3a38f96a1fa01406def71a3517894a.pdf
Al-Azhar University, Faculty of Science (Boys)
Al-Azhar Bulletin of Science
1110-2535
2636-3305
23
Issue 2-C
2012
12
01
SURVEY OF SOME PLANT EXTRACTS AGAINST CERTAIN HUMAN FUNGAL PATHOGENS
13
32
EN
AL-SAYED
A.
RAGAB
Plant & Microbiology Department, Faculty of Science,(Girls' Branch), Al-Azhar University, Cairo, Egypt.
H.
H.
EL- SHIEKH
Plant & Microbiology Department, Faculty of Science,( Boys' Branch), Al-Azhar University, Cairo, Egypt.
EMAN
M.
EL-TAHER
The Regional Center for Mycology and Biotechnology (RCMB), Al-Azhar University, Cairo, Egypt.
BASMA
H.
AMIN
The Regional Center for Mycology and Biotechnology (RCMB), Al-Azhar University, Cairo, Egypt.
10.21608/absb.2012.7211
<span style="font-size: small;"><span style="font-size: small;">Antifungal agents (A.f.A.) play an important role in the treatment of human serious diseases. Therefore, A.f.A. from natural sources was preferred than that synthetic compounds. Methanol, methanol: water; (1:1), water, chloroform and benzene extracts of </span><span style="font-size: small;">thirty two plant organs; sixteen desert and sixteen cultivated plant organs</span><span style="font-size: small;"> were assessed against forty six human fungal pathogenic isolates; twelve (yeast), two (yeast like fungi) and thirty two (filamentous fungi).</span><span style="font-size: small;">From the five assessed extracts, chloroform extracts exhibit the most significant activity followed by benzene, methanol: water; (1:1) and methanol, while, water extracts exhibited the least activity. Chloroform extracts revealed the highest inhibitory effect against the mycelial growth of the human fungal isolates. </span><span style="font-size: small;"><em><strong>Thymus serpyllum</strong></em></span><span style="font-size: small;"><span lang="en-GB"><strong> L. </strong></span></span><span style="font-size: small;"><span lang="en-GB">(desert plant) and</span></span><span style="font-size: small;"><span lang="en-GB"><em><strong>Anethum graveolens </strong></em></span></span><span style="font-size: small;"><span lang="en-GB">(cultivated plant) were active against </span></span><span style="font-size: small;"><em><strong>Aspergillus tamarii</strong></em></span><span style="font-size: small;"><strong> 1 </strong></span><span style="font-size: small;">and</span><span style="font-size: small;"><em><strong>Penicillium marneffei</strong></em></span><span style="font-size: small;">isolated from eyes and</span><span style="font-size: small;">blood, respectively. </span><span style="font-size: small;">Scientifically, this study recommended the use of these plant extracts as a potent antifungal agent after their purification and identification.</span></span>
antifungal agents,Plant extracts,human pathogenic fungi
https://absb.journals.ekb.eg/article_7211.html
https://absb.journals.ekb.eg/article_7211_18883c5af0d3934428e1927785ac97f5.pdf
Al-Azhar University, Faculty of Science (Boys)
Al-Azhar Bulletin of Science
1110-2535
2636-3305
23
Issue 2-C
2012
12
01
ISOLATION AND CHARACTERIZATION OF NOVEL EXTREMELY HALOTHERMOPHILIC BACTERIUM, HALOMONAS CASEINILYTICA WN.1B.S, FROM WADI AN NATRUN, EGYPT
33
48
EN
S.
S.
EL-LOUBOUDY
Botany and Microbiology department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt
M.
S.
EL-GAMAL
Botany and Microbiology department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt
H.
M.
MAHDY
Botany and Microbiology department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt
M.
G.
MOHAMED
Botany and Microbiology department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt
10.21608/absb.2012.7212
<span style="font-size: small;"><span style="font-size: small;">The purpose of the present study was to isolate microbial halothermophiles from hyper saline Al</span><span style="font-size: small;">- Hamra Lake</span><span style="font-size: small;">at</span><span style="font-size: small;"> Wadi An Natrun</span><span style="font-size: small;">, Egypt</span><span style="font-size: small;">. Twenty </span><span style="font-size: small;">e</span><span style="font-size: small;">ight bacterial isolates were obtained</span><span style="font-size: small;"> and the</span><span style="font-size: small;"> morphological and physiological properties in addition to enzyme activities were studied. </span><span style="font-size: small;">Amongst</span><span style="font-size: small;"> those isolates, </span><span style="font-size: small;">WN.1B.s</span><span style="font-size: small;"> was selected as the m</span><span style="font-size: small;">ost potent isolate based on </span><span style="font-size: small;">growth</span><span style="font-size: small;"> at high temperature </span><span style="font-size: small;">(</span><span style="font-size: small;">up to 65</span><sup><span style="font-size: small;">o</span></sup><span style="font-size: small;">C</span><span style="font-size: small;">)</span><span style="font-size: small;"> and </span><span style="font-size: small;">at high </span><span style="font-size: small;">salt concentration </span><span style="font-size: small;">(</span><span style="font-size: small;">up to 34%</span><span style="font-size: small;">,</span><span style="font-size: small;"> near saturation state).</span><span style="font-size: small;">A phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate WN.1B.s had the highest sequence similarity with respect to type strains of </span><span style="font-size: small;"><em>Halomonas caseinilytica</em></span><span style="font-size: small;"> (97 %)</span><span style="font-size: small;">, </span><span style="font-size: small;"><em>Halomonas elongata</em></span><span style="font-size: small;"> (96 %), </span><span style="font-size: small;"><em>Halomonas eurihalina</em></span><span style="font-size: small;"> (95 %), </span><span style="font-size: small;"><em>Halomonas koreensis</em></span><span style="font-size: small;"> (95 %) and </span><span style="font-size: small;"><em>Halomonas halmophila</em></span><span style="font-size: small;"> (95 %).</span><span style="font-size: small;">B</span><span style="font-size: small;">ased on physiological </span><span style="font-size: small;">characteristics</span><span style="font-size: small;"> and</span><span style="font-size: small;">16S rRNA sequence analysis</span><span style="font-size: small;">t</span><span style="font-size: small;">his isolate</span><span style="font-size: small;"> was identified </span><span style="font-size: small;">as </span><span style="font-size: small;"><em>Halomonas caseinilytica</em></span><span style="font-size: small;">WN.1B.s</span><span style="font-size: small;"> which belonged to</span><span style="font-size: small;"> bacterial domain, class </span><span style="font-size: small;"><em>Gammaproteobacteria</em></span><span style="font-size: small;">, order </span><span style="font-size: small;"><em>Oceanospirillales</em></span><span style="font-size: small;">, </span><span style="font-size: small;"><em> family Halomonadaceae, Halomonas species.</em></span><span style="font-size: small;"> Enzyme screening for strain WN.1B.s showed that, the isolate secrete amylases, lipases, cellulases and pectinase enzymes under harsh conditions that may be useful in different industrial processes.</span></span>
HALOTHERMOPHILIC BACTERIUM,HALOMONAS CASEINILYTICA WN.1B.S,,WADI AN NATRUN,Egypt
https://absb.journals.ekb.eg/article_7212.html
https://absb.journals.ekb.eg/article_7212_a9f7827a7aa05efd70480018bccd964b.pdf
Al-Azhar University, Faculty of Science (Boys)
Al-Azhar Bulletin of Science
1110-2535
2636-3305
23
Issue 2-C
2012
12
01
DISTRIBUTION AND ABUNDANCE OF ZOOPLANKTON IN THE RIVER NILE,EGYPT
49
60
EN
NEHAD
K.
SAYED
National Institute of Oceanography and Fisheries, El Qanater Elkairia, Egypt.
KHADIGA
M.
GABER
National Institute of Oceanography and Fisheries, El Qanater Elkairia, Egypt.
FATEN
A.
ELFEKY
Faculty of Science, Girls Branch, Al-Azhar University, Cairo, Egypt.
10.21608/absb.2012.7213
<span style="font-size: small;"><span style="font-size: small;">Zooplankton is known to form an important component of the biological communities in large rivers due to their high abundance and ability to cycle nutrients through the aquatic environment</span><span style="font-size: small;">. Little is known about these communities in the River Nile, so the present study was designed on sampling</span><span style="font-size: small;">Zooplankton in the River Nile (main body) and its two branches, Rosetta and Damietta, along four seasons of the year 2009. During the investigation period, </span><span style="font-size: small;">Rotifera represented the most dominant species recording 54% followed by Protozoa, Cladocera, Copepoda, and Meroplankton with ratios of 17%, 15%, 9%, and 6% respectively.</span><span style="font-size: small;"> There were marked seasonal differences between individual species in the main body of the river and its two branches.</span></span>
Zooplankton,River Nile,,EGYPT
https://absb.journals.ekb.eg/article_7213.html
https://absb.journals.ekb.eg/article_7213_2613f12d47499ca165cf38bc9038e6b9.pdf
Al-Azhar University, Faculty of Science (Boys)
Al-Azhar Bulletin of Science
1110-2535
2636-3305
23
Issue 2-C
2012
12
01
VARIATION OF PROTEIN PROFILE AMONG CONSECUTIVE STINGS OF THE SCORPION PARABUTHUS LEIOSOMA (FAMILY: BUTHIDAE) FROM EGYPT, SUPPORTS THE VENOM-METERING HYPOTHESIS IN SCORPIONS.
61
72
EN
MOUSTAFA
M.
SARHAN
Zoology Department, Faculty of Science, Al Azhar University, Assiut, Egypt.
MAGED
M.
FOUDA
Zoology Department, Faculty of Science, Al Azhar University, Assiut, Egypt.
ALAA
M.
ELBITAR
Zoology Department, Faculty of Science, Al Azhar University, Assiut, Egypt.
HAMDY
A.
ALY
Zoology Department, Faculty of Science, Al Azhar University, Assiut, Egypt.
AHMED
B.
SAYED
Zoology Department, Faculty of Science, Al Azhar University, Cairo, Egypt.
10.21608/absb.2012.7209
<span style="font-size: small;"><span style="font-size: small;">Scorpions use their venom to immobilize prey items and defend themselves against predators. Scorpion venom is a complex mixture composed of a wide array of substances such as salts, small molecules, peptides, and proteins. During analysis of the prey capture behavior of the scorpion </span><span style="font-size: small;"><em>Parabuthus leiosoma,</em></span><span style="font-size: small;"> we noticed that the scorpion stings the prey several successive stings in different specific regions. The stings started with ventral surface of the abdomen followed by thorax, shoulders and finally the head. This finding raised the possibility that the scorpion might use the venom with different composition in different contexts. To check this possibility, venom was collected as sequential drops (stings) by electrical stimulation (20 Volt) of the telson of the </span><span style="font-size: small;"><em>Parabuthus leiosoma</em></span><span style="font-size: small;">. Protein content was determined by spectrophotometer using total protein KIT. The results showed marked variation of the total protein among the consecutive stings of each of the investigated scorpions. To go further, the protein profiles for all the collected stings were analyzed separately using 10% SDS-polyacrylamide electrophoresis (SDS-PAGE). Similarly, differences were observed among the stings, not only in the number of the protein bands, but also in the intensity of the bands. Thus, these results indicated that different venom components are unequally represented among consecutive stings supporting the venom-metering hypothesis. </span></span>
Scorpion,Venom,Stinging,Consecutive stings,Electrophoresis,Parabuthus leiosoma
https://absb.journals.ekb.eg/article_7209.html
https://absb.journals.ekb.eg/article_7209_128c99eb15f0764fa43912f3d5354480.pdf
Al-Azhar University, Faculty of Science (Boys)
Al-Azhar Bulletin of Science
1110-2535
2636-3305
23
Issue 2-C
2012
12
01
EFFECT OF ALTITUDINAL VARIATIONS ON THE DISTRIBUTION OF NEPETA SEPTEMCRENATA IN SAINT KATHERINE PROTECTORATE, SOUTH SINAI, EGYPT.
73
80
EN
M.
MANSOUR
Botany Department, Faculty of Science (Boys branch),Al-Azhar University, Cairo, Egypt.
K.
OMAR
Saint Katherine Protectorate, the Nature Conservation Sector (NCS), Egyptian Environmental Affairs Agency (EEAA).
10.21608/absb.2012.7214
<span style="font-size: small;"><span style="color: #000000;"><span style="font-size: small;">A study was carried out on a wild herb </span></span><span style="font-size: small;"><em>Nepeta septemcrenata </em></span><span style="font-size: small;">Benth. </span><span style="color: #000000;"><span style="font-size: small;">(Family : Lamiaceae) in </span></span><span style="font-size: small;">Saint Katherine Protectorate, South Sinai, Egypt</span><span style="color: #000000;"><span style="font-size: small;"> to assess the effect of altitude (1400 to 2200 m above mean sea level) on plant distribution, morphological parameters, soil component and grazing pressure. The results show that elevation influence on physical and chemical properties of soil. Water content show great variation among different elevation ranks. Plant traits also affected by elevation gradient. Results found that </span></span><span style="color: #000000;"><span style="font-size: small;"><em>N.septemcrenata</em></span></span><span style="color: #000000;"><span style="font-size: small;"> cover, Importance value, Number of individuals and internodes length increased positively with elevation while species richness decreases. Plant width, height and No. of branches also affected by elevation gradient and show highest values at elevation between 2000-2200 m.</span></span></span>
Nepeta septemcrenata,Saint Katherine Protectorate,altitudinal variation
https://absb.journals.ekb.eg/article_7214.html
https://absb.journals.ekb.eg/article_7214_14cc14a1594c1587995042729ae63b1f.pdf
Al-Azhar University, Faculty of Science (Boys)
Al-Azhar Bulletin of Science
1110-2535
2636-3305
23
Issue 2-C
2012
12
01
INFLUENCE OF TOPOGRAPHIC ASPECT ON THE DISTRIBUTION OF NEPETA SEPTEMCRENATA IN SAINT KATHERINE PROTECTORATE, SOUTH SINAI, EGYPT.
81
91
EN
M.
MANSOUR
Botany Department,Faculty of Science (Boys branch), Al-Azhar University, Cairo, Egypt.
K.
OMAR
Saint Katherine Protectorate, the Nature Conservation Sector (NCS), Egyptian Environmental Affairs Agency (EEAA).
10.21608/absb.2012.7215
<span style="font-size: small;"><span style="color: #000000;"><span style="font-size: small;">A study was carried out on a wild herb </span></span><span style="font-size: small;"><em>Nepeta septemcrenata </em></span><span style="font-size: small;">Benth. </span><span style="color: #000000;"><span style="font-size: small;">(Family: Lamiaceae) in </span></span><span style="font-size: small;">Saint Katherine Protectorate, South Sinai, Egypt</span><span style="color: #000000;"><span style="font-size: small;"> to assess the effect of topographic aspect on plant distribution, morphological parameters and soil component. The results show that aspect influence on physical and chemical properties of soil. Water content showed great variation among different aspect directions. Plant traits also affected by these directions. Results found that 26.8% of </span></span><span style="color: #000000;"><span style="font-size: small;"><em>N.septemcrenata</em></span></span><span style="color: #000000;"><span style="font-size: small;"> populations’ recorded at North aspect and no observation for </span></span><span style="color: #000000;"><span style="font-size: small;"><em>Nepeta</em></span></span><span style="color: #000000;"><span style="font-size: small;"> at South aspect. Soil physical properties showed significant variation among different aspect ranks. Results also showed positive correlation between soil total dissolved solids, </span></span><span style="font-size: small;">electrical conductivity</span><span style="color: #000000;"><span style="font-size: small;">, sodium, potassium and chloride. This component show highest degree at east aspect. Soil pH and </span></span><span style="color: #231f20;"><span style="font-size: small;">calcium carbonate showed the highest value at south west aspect, while calcium and magnesium show highest value at North east aspect.</span></span></span>
Nepeta septemcrenata,Saint Katherine Protectorate,aspect,Topography,spatial variation
https://absb.journals.ekb.eg/article_7215.html
https://absb.journals.ekb.eg/article_7215_079878933ad09dc5e8d78d0f6a275a14.pdf