ORIGINAL_ARTICLE
PROTECTIVE AND THERAPEUTIC EFFECTS OF OLIVE OIL AND FICUS CARICA AS NATURAL ANTIOXIDANTS ON SOME BIOCHEMICAL PARAMETERS IN LIVER OF γ-IRRADIATED MALE ALBINO RATS
The present study was designed to determine the possible protective and therapeutic effects of olive oil, Ficus carica andboth against radiation induced oxidative stress and biochemical changes in liver of albino rats. A patch of 114 male Wisteralbino rats averaged weights 120±5 g at the beginning of the experiment were divided into 8 main groups and 19 subgroups according to the treatment and requirements of the experiment. Irradiation was performed by whole body exposure of rats to an acute single dose gamma radiation of 6 Gy. Irradiated rats received, via gavage, extra virgin olive oil (7.6 ml/kg b.wt), extract of Ficus carica fruit (1 g/kg b.wt) and the combined supplementation of both Ficus carica (1 g/kg b.wt) and extra virgin olive oil (7.6 ml/kg b.wt) before and/or after radiation exposure. Six rats were sacrificed on the 1st and 15th day post irradiation exposure at the control, treated and pretreated irradiated subgroups. In addition, six rats were sacrificed from the post-treated irradiated subgroups on the 15th day post irradiation exposure.In the present study, γ- irradiation induced dramatic results of all parameters and sever antioxidant and detoxification enzyme depletion. Exposure to γ- radiation resulted in a significant elevation of some hepatic parameters (TBARS, AOPP, SOD, ASAT, ALAT, and ALP) and a significant decrease in some other parameters (CAT, GSH and NO). Moreover, the present study showed a significant decrease in total protein, and albumin on the 1st and 15th day post-irradiation as compared to the control. The administration of the olive oil or Ficus carica or both had a beneficial result against the deleterious effects of γ-irradiation. In addition, all treatments showed a significant increase in TBARS and AOPP levels, enhance SOD and CAT activities, and improve GSH and NO concentrations in the liver homogenate. Furthermore, significant amelioration in the serum liver function enzymes activities (ASAT, ALAT and ALP), total protein, and albumin were observed in comparison to irradiated rats. A better ameliorative effect was noticed with the combined extra virgin olive oil and Ficus carica treatments that revealed the synergistic effect between them. In conclusion, according to the results obtained the administration of the olive oil or Ficus carica or both provides considerable radioprotective and radiotherapeutic effects against whole body γ- radiation in male Wister albino rats by preventing oxidative stress through ROS scavenger.
https://absb.journals.ekb.eg/article_25184_d41d8cd98f00b204e9800998ecf8427e.pdf
2014-06-01
1
16
10.21608/absb.2014.25184
olive oil
Ficus carica
γ- radiation
Oxidative Stress
antioxidants
Mohamed
Bashandy
1
Departments of aZoology, Faculty of Science, Al-Azhar University, Cairo, Egypt
LEAD_AUTHOR
Hesham
Abd El- Rasheid
2
Departments of aZoology, Faculty of Science, Al-Azhar University, Cairo, Egypt.
AUTHOR
Hesham
Hasan
3
Radiation biology, National Center for Radiation Research and Technology, Atomic energy authority, Cairo, Egypt.
AUTHOR
Abdallah
Fathya
4
Departments of aZoology, Faculty of Science, Al-Azhar University, Cairo, Egypt.
AUTHOR
ORIGINAL_ARTICLE
THE RELATIONSHIP BETWEEN INSULIN RESISTANCE AND HEPATITIS C VIRUS INFECTION IN EGYPTIAN PATIENTS
Background: Chronic hepatitis C virus (HCV) infection is one of the most common problems in Egypt. The prevalenceof diabetes mellitus is associated with HCV infection, it has a relation with Homeostatic Model Assessment insulin resistance (HOMA-IR).Objective: To investigate the impacts of HCV infection (with and without cirrhosis) on glucose metabolism and its relationifany-with body mass index (BMI) in chronic HCV patients.Patients and Methods: Sixty cases were involved in this study, they were classified into three groups; group I: 20 healthycontrol, group II: 20 chronic HCV patients without cirrhosis and group III: 20 chronic HCV patients with cirrhosis. All groups were subjected to the following: full history taking, through clinical examination, fasting and post prandial blood glucose levels (FBG and PPG), assessment of fasting plasma insulin level was done by the immune-enzymatic method. Assessment of the insulin resistance state was done by Homeostatic Model Assessment (HOMA-IR) and calculation of BMI. In addition, liver function tests (ALAT, ASAT, ALP, total bilirubin, albumin, and prothrombin time) and Detection of anti-HCV was done by the 3rd generation ELISA test and confirmed by qualitative polymerase chain reaction (PCR).Results: Cirrhotic patients were found to have significantly higher fasting plasma insulin levels, insulin resistance (HOMAIR), blood glucose (FBG and PPG) and lower synthetic liver functions (albumin and prothrombin time) than the control and non-cirrhosis groups. Cirrhotic and non-cirrhotic patients were found to have significantly higher serum bilirubin, ALAT and ASAT as compared to the control. However, non-cirrhotic patients were found to have significantly higher alkaline phosphatase (ALP) than the control and cirrhosis groups.Conclusion: Chronic HCV infection with cirrhosis may be regarded as an independent risk factor for the development ofinsulin resistance and type 2 diabetes. HCV with cirrhosis induces insulin resistance, the key step for glucose intolerance. The impacts of chronic HCV infection with cirrhosis on glucose metabolism should be recognized in clinical care centers and addressed in future studies.
https://absb.journals.ekb.eg/article_25185_d41d8cd98f00b204e9800998ecf8427e.pdf
2014-06-01
17
26
10.21608/absb.2014.25185
Hepatitis C virus
HCV
Cirrhosis
diabetes
HOMA-IR
BMI
Samir
Zaahkouk
1
Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.
LEAD_AUTHOR
Olfat
Hendy
2
Clinical Pathology Department, National Liver Institute, Monoufiya University, Egypt.
AUTHOR
Hesham
Abd El- Rasheid
3
Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.
AUTHOR
Gamal
Allaithy
4
Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Effects of nitrate and ph sphate on the growth and total lipid contents of Chlorella oocystoides and Chlorella minutissima ( Chlorophyceae)
The effects of different concentrations (0.1, 0.5, 1.5 and 4.5 ppm) of nitrate (NO3-) as NaNO3 and phosphate ( PO4---) asK2HPO4 on growth parameters (dry weight, optical density, total pigments), total lipid, total carbohydrate and total protein contents of Chlorella oocystoides and Chlorella minutissima cultured were followed for 7 days.The growth parameters (dry weight, optical density and total pigments), total carbohydrate and total protein contents ofChlorella oocystoides and Chlorella minutissima were significantly increased up to the concentration 1.5 ppm of NO3- andPO4--- Under relatively higher concentration 4.5 ppm of NO3- and PO4--- all these parameters were significantly decreased. On the contrary, the total lipid contents were significantly increased up to the 0.1 ppm of NO3- and PO4--- However, under relatively higher concentration 4.5 ppm of NO3- and PO4---, the total lipid contents were significantly decreased
https://absb.journals.ekb.eg/article_25190_d41d8cd98f00b204e9800998ecf8427e.pdf
2014-06-01
27
43
10.21608/absb.2014.25190
Chlorella
growth parameters
total pigment and total lipid contents
S.
Desouky
1
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Assiut, Egypt.
AUTHOR
Taher
Taha
2
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Assiut, Egypt.
AUTHOR
Hafez
Elsayed
3
Plant Molecular Pathology Department. City for Scientific Research and Technology Applications, New Borg El-Arab, Alexandria, Egypt.
AUTHOR
Abdel- Mola
Mebed
4
External student higher studies, Faculty of Science, Al-Azhar University, Assiut, Egypt.
AUTHOR
ORIGINAL_ARTICLE
MOLECULAR ANALYSIS OF INTERACTION PARTNERS OF THE TRANSCRIPTIONAL REPRESSOR BLIMP-1 IN DROSOPHILA MELANOGASTER BY A PROTEOMICS APPROACH.
B lymphocyte induced maturation protein (Blimp-1) is present in a variety of organisms from nematode to human as a factor carrying well conserved five DNA binding zinc fingers and relatively conserved PR/SET domain. Blimp-1 was found to play important roles in various aspects of development in many organisms andworks mainly as a repressor to suppress many different genes. Blimp-1 inDrosophila was identified as a transcriptional repressor binds to the promoter region of the ftz-f1 gene. To understand the mechanism of how Drosophila Blimp-1 controls expression of its target genes, Blimp-1 was purified from prepared nuclear extract with its interacting proteins. The purified proteins were identified using Liquid chromatography-mass spectrometry (LC-MS/MS) after trypsin digestion. Results of this analysis revealed presence of several peptides of Histone H4 as interacting protein. It could be suggested that Blimp-1 function through a histone-masking mechanism and has an important regulatory chromatin remodeling.
https://absb.journals.ekb.eg/article_25193_d41d8cd98f00b204e9800998ecf8427e.pdf
2014-06-01
35
42
10.21608/absb.2014.25193
Drosophila
Blimp-1
Histone H4
Transcription factor
Gene regulation
Moustafa
Sarhan
1
Zoology Department, Faculty of Science, Al Azhar, University, Assiut
LEAD_AUTHOR
ORIGINAL_ARTICLE
EFFECT OF HEAVY METALS ON SOME PHYSIOLOGICAL RESPONSES IN TWO FISH SPECIES INHABITING MEDITERRANEAN SEA COAST; DAMIETTA GOVERNORATE, EGYPT.
The present study aims to determine the concentrations of heavy metals: Cadmium, Copper, Iron, Nickel, Lead and Zincin the two fish species, Sparus aurata and Diplodus sargus, (family: Sparidae) collected from the Mediterranean Sea Coast, Damietta Governorate, in order to: compare concentrations of metals in the different organs (gonads, kidney, liver, muscles, gills, skin and bones) and their effects on the physiological parameters (total proteins, total lipids, ASAT and ALAT) in the edible organs of these species. Results revealed that, the highest values of heavy metals in the different organs of S. aurata and D. sargus were recorded during summer. Although, the two species have the same behavior of feeding, S. aurata showed high values of the different metals than D. sargus. Biochemical analysis indicated devastating effects in metabolic parameters and enzymes activities in S. aurata than D. sargus. ANOVA (p> 0.05) showed significant differences between the different organs and metals. Also, biochemical parameters exhibited a significant increase between seasons and the different organs and a slight difference between the studied fishes.
https://absb.journals.ekb.eg/article_26039_aaf88e76544b016470d2d7c5e1d4a7a6.pdf
2014-06-10
43
56
10.21608/absb.2014.26039
Heavy metals
biochemical parameters
Sparus aurata
Diplodus sargus
Mohamed
Ghanem
1
Department of Zoology, Faculty of Science, Al-Azhar University.
LEAD_AUTHOR
ORIGINAL_ARTICLE
EFFECT OF GAMMA IRRADIATION ON CELLULASE ACTIVITY OF PLEUROTUS PULMONARIUS DURING THE DIFFERENT GROWTH STAGES ON DIFFERENT PLASTIC POLYETHYLENE WASTES
Pleurotus pulmonarius was excellent growth on determined weights of the wet rice straw, non irradiated plastic, irradiatedplastic, mixture of rice straw and non irradiated plastic and mixture of rice straw and irradiated plastic. maximum value ofcellulase recorded on rice straw, mixture of rice straw and irradiated plastic, mixture of rice straw and non- irradiated plastic (1.7, 1.5 and 1.3 unit/gm waste), respectively, which recorded after first harvest; but irradiated plastic and non-irradiated plastic giving highest values at promordium stage; (1.14 and 0.79 unit/gm waste). The spawn of P. pulmonarius was irradiated at the does 0.5, 1 and 2 KGy, and inoculated on each different wastes separately to detect the cellulase activity which was increased in the various wastes by exposing the spawn of P. pulmonarius to gamma irradiation and detectable raises in the cellulase activity was recorded at dose 0.5 KGy, Any further increase in theirradiation dose was accompanied by a decrease in cellulase activity until reach lowest value at 2 KGy.
https://absb.journals.ekb.eg/article_26042_3e5af3064c0ed65ad7e8d6bcc7770f9a.pdf
2014-06-01
57
64
10.21608/absb.2014.26042
B.
Hashem
1
Microbiology department,Faculty of Science ,Ain shamsUniversity,Egypt
LEAD_AUTHOR
F.
EL-Beih
2
Microbiology department,Faculty of Science ,Ain shams University, Egypt
AUTHOR
S.
Abd El-Aziz
3
Radio-Biochemistry, Atomic Energy Authority, Egypt
AUTHOR
S.
Easanin
4
Microbiology department, Faculty of Science ,Ain shams University,Egypt
AUTHOR
H.
EL-Halby
5
Microbiology department, Middle Eastern Regional Radioisotope center for the Arab Countries, Egypt.
AUTHOR
ORIGINAL_ARTICLE
MONITORING OF DRINKING WATER QUALITY BY BACTERIAL INDICATORS AND TRIAL FOR DETECTION
In this study eight water samples were collected from three plants, (Al-Arida plant, (3 sample),Shobra-blola plant (3sample), and Meet- maemon plant (2 sample) from AL-Gharbia, Egypt .The results showed that the seasonal variation inheterotrophic and streptococcus bacteria showed the microbial prevalence in raw water of Al-Arida plant, but not detected in filtrated water. On the other hand ,both of total and fecal Coliform bacteria showed microbial prevalence in raw water of Al-Arida plant mean while disappeared in filtrated water. However, Shobra-blola plant and Meet- maemon plant showed seasonal variation in heterotrophic and streptococcus bacteria in a permissible limits. Amazingly new media (Lauryl tryptose broth with mug) has the ability to detect the indicator bacteria in only 24 hr (s). versus conventional methods as Most Probable Number (MPN) and membrane filtration (MF). Accordingly , the pollution level have been indicated as water quality index ,(WQI) for all annual seasons which its arrangement was in descending order as , Winter < Autumn < Spring < Summer respectively .Also, the highest Pollution levels was in winter season as compared with other seasons. This phenomenon may be attributed to the accumulation of wastes of drains which is accompanied by winter stagnant period while lowers water level in River Nile leading to an increase in pollutants loaded and decreased in diluted levels.
https://absb.journals.ekb.eg/article_26046_5e9e7d9c650000a62aace4c5004dd4af.pdf
2014-06-27
65
76
10.21608/absb.2014.26046
Drinking water
bacterial indicators
Detection trials
total & fecal Coliform bacteria
A.
Farrag
1
Al-Azhar University. Faculty of science, microbiology Department.Nassar City, Cairo, Egypt
LEAD_AUTHOR
A.
Khalil
2
Al-Azhar University. Faculty of science, microbiology Department.Nassar City, Cairo, Egypt.
AUTHOR
M.
El.Bahnasy
3
Water and waste Water Company .Central lab, El-Gharbia Gavernate.Tanta, Egypt.
AUTHOR