ORIGINAL_ARTICLE
A HIGH POTENT ANTIBODY RESPONSE IN RABBITS INFECTED WITH ULTRA VIOLET ATTENUATED CERCARIAE
Ultraviolet (UV) light is an electromagnetic radiation below the visible wavelengths. Their use in cercariae attenuation needs to be examined. In the present work, Schistosoma mansoni cercariae was exposed to UV radiation. Few studies showed previously an inflicted damage seen on the adult schistosome worms developed from irradiated cercariae. The aim of the study was to find out whether this damage was attributed to direct effect of UV-irradiation on cercariae or due to the host's immunogenicity induced by UV- irradiated cercariae. Single as well as multiple exposures of cercariae to UV Light were examined. They were exposed to UV light, for 1, 2 and 3 hrs and then, were subjected to different treatments: the first one was for the assessment of cercarial viability after one hour of the 3 different treatments. The second one was for rabbit infection and the third one was for cercarial antigen preparation. The cercarial antigens (Ag) were recognized by protective antibodies (IgG1 fractions) which then, were separated and purified from vaccinated rabbit's serum. This cercarial Ag was identified as UVISmC1 gene encoding a protein showing 100% identity at the amino acid level with previously identified S. mansoni clones; theses clones are encoding 51.7 kDa antigens elicited as a result of direct effect of UV radiation on cercariae as well as host's immunogenicity induced by UV irradiated cercariae. This was verified by the recognition of this Ag prepared from E coli clones isolated from cDNA expression library. In conclusion, the data showed a remarkable potency of the UV-radiation-attenuated cercaria in eliciting differential high effectiveness in Ab response under laboratory conditions at 1, 2 and 3 hrs of UV light output at 254 nm. An understanding of the protective immune response elicited by RA cercaria may help in designing a candidate vaccine which is still needed.
https://absb.journals.ekb.eg/article_7029_e9c604b7755e20855a893243414db44f.pdf
2011-12-01
1
16
10.21608/absb.2011.7029
UV light
Schistosoma mansoni
radiation-attenuated cercariae
purification of IgG and vaccine
HALA
MOUSTAFA
1
Medical Biophysics Department, Faculty of Applied Medical Science, 6 October University, 6 October, Egypt.
LEAD_AUTHOR
SAMAR
HASSAB-ALLAH
2
Biochemistry Department, Faculty of Physical Therapy, 6 October University, 6 October City, Egypt
AUTHOR
HARBI
ALI
3
Biology Department, Faculty of Science, Jazan University, Saudi Arabia
AUTHOR
HANAN
RABEE
4
Narcotic Department, National Center for Social and Criminological Research, Cairo, Egypt.
AUTHOR
RAGAA
ALI
5
Biology Department Basic Science, 6 October University, 6 October City, Egypt.
AUTHOR
ORIGINAL_ARTICLE
REPRODUCTIVE BIOLOGY OF THE FLATTENED PORCELAIN CRAB, PETROLISTHES RUFESCENS (PORCELLANIDAE: ANOMURA: CRUSTACEA) FROM AIN SUKHNA, GULF OF SUEZ, EGYPT
The reproductive biology of the porcelain crab Petrolisthes rufescens(Heller, 1861) population at Ain Sukhna (62 km south Suez City) was studied during the period from April 2000 to May 2001. Sexual dimorphism was determined based on morphological differences between sexes. Abdomen is semi-circular with four pairs of biramous appendages in females, but tapering with only two pairs of unequal uniramous appendages (pleopds) in males. Size at first maturity was determined using allometric growth occurring at puberty from the relationships between male's 2nd pleopodal length and chelae length against carapace length (C.L.) and by the appearance of distinctive ovaries in females. Overall sex ratio for this crab species was 1: 0.70 males to females, with highly significant Chi-square value (X2 = 7.486, P< 0.05, df =1).It was not constant throughout the year particularly during breeding seasons, showing highly significant Chi-square value (X2 = 20.185, df= 25, P < 0.05). Based on the appearance of ovigerous females, the breeding of P. rufescens is lengthy, start often at late winter and early spring and extends through summer to early autumn. Incubated eggs are oval or semi-oval, sometimes elliptical with polygonal surfaces, and varied in size from 400 to 1000 m with an average of 640 ± 105 m. These eggs are passed through five stages of maturation, and showed remarkably gradual decreasing in size throughout the period of incubation, reaching the minimum at last stages (stage V or the releasing larvae). The color of incubated eggs is also changed from bright yellow in newly laid eggs to grey at the last stage of development. The fecundity of ovigerous females was relatively low, varied from 35 to 450 with an average of 208 ± 92 eggs /female crab. It was correlated by a curvilinear relationship with carapace length only.
https://absb.journals.ekb.eg/article_7030_060f4d17f1ac810660e953a522c39b1b.pdf
2011-12-01
17
33
10.21608/absb.2011.7030
PORCELLANIDAE
ANOMURA
Crustacea
Gulf of Suez
AWAAD
EL-SAYED
1
Section of Marine Biology and Fishes, Zoology Department, Faculty of Science, Al- Azhar University, Cairo, Egypt.
LEAD_AUTHOR
MOSTAFA
FOUDA
2
Consultant for Minister of Environment, State Ministry of Environment, Cairo, Egypt.
AUTHOR
AHMED
AZAB
3
Section of Marine Biology and Fishes, Zoology Department, Faculty of Science, Al- Azhar University, Cairo, Egypt.
AUTHOR
ISMAIL
ISMAIL
4
M.Sc. in Marine Biology.
AUTHOR